21131488
not annotated - annotated - LINNAEUS only
Bacillus anthracis sin locus and regulation of secreted proteases.
Bacillus anthracis shares many regulatory loci with the nonpathogenic Bacillus species Bacillus subtilis. One such locus is sinIR, which in B. subtilis controls sporulation, biofilm formation, motility, and competency. As B. anthracis is not known to be motile, to be naturally competent, or to readily form biofilms, we hypothesized that the B. anthracis sinIR regulon is distinct from that of B. subtilis. A genome-wide expression microarray analysis of B. anthracis parental and sinR mutant strains indicated limited convergence of the B. anthracis and B. subtilis SinR regulons. The B. anthracis regulon includes homologues of some B. subtilis SinR-regulated genes, including the signal peptidase gene sipW near the sinIR locus and the sporulation gene spoIIE. The B. anthracis SinR protein also negatively regulates transcription of genes adjacent to the sinIR locus that are unique to the Bacillus cereus group species. These include calY and inhA1, structural genes for the metalloproteases camelysin and immune inhibitor A1 (InhA1), which have been suggested to be associated with virulence in B. cereus and B. anthracis, respectively. Electrophoretic mobility shift assays revealed direct binding of B. anthracis SinR to promoter DNA from strongly regulated genes, such as calY and sipW, but not to the weakly regulated inhA1 gene. Assessment of camelysin and InhA1 levels in culture supernates from sinR-, inhA1-, and calY-null mutants showed that the concentration of InhA1 in the culture supernatant is inversely proportional to the concentration of camelysin. Our data are consistent with a model in which InhA1 protease levels are controlled at the transcriptional level by SinR and at the posttranslational level by camelysin.
Ann file
T1 Species 0 18 Bacillus anthracis
N1 Reference T1 Taxonomy:1392
T2 Species 68 86 Bacillus anthracis
N2 Reference T2 Taxonomy:1392
T3 Species 155 172 Bacillus subtilis
N3 Reference T3 Taxonomy:1423
T4 Species 208 219 B. subtilis
N4 Reference T4 Taxonomy:1423
T5 Species 290 302 B. anthracis
N5 Reference T5 Taxonomy:1392
T6 Species 411 423 B. anthracis
N6 Reference T6 Taxonomy:1392
T7 Species 463 474 B. subtilis
N7 Reference T7 Taxonomy:1423
T8 Species 524 536 B. anthracis
N8 Reference T8 Taxonomy:1392
T9 Species 607 619 B. anthracis
N9 Reference T9 Taxonomy:1392
T10 Species 624 635 B. subtilis
N10 Reference T10 Taxonomy:1423
T11 Species 655 667 B. anthracis
N11 Reference T11 Taxonomy:1392
T12 Species 704 715 B. subtilis
N12 Reference T12 Taxonomy:1423
T13 Species 837 849 B. anthracis
N13 Reference T13 Taxonomy:1392
T14 Species 963 978 Bacillus cereus
N14 Reference T14 Taxonomy:1396
T15 Species 1170 1179 B. cereus
N15 Reference T15 Taxonomy:1396
T16 Species 1184 1196 B. anthracis
N16 Reference T16 Taxonomy:1392
T17 Species 1277 1289 B. anthracis
N17 Reference T17 Taxonomy:1392