21148728
not annotated - annotated - LINNAEUS only
Three motAB stator gene products in Bdellovibrio bacteriovorus contribute to motility of a single flagellum during predatory and prey-independent growth.
The predatory bacterium Bdellovibrio bacteriovorus uses flagellar motility to locate regions rich in Gram-negative prey bacteria, colliding and attaching to prey and then ceasing flagellar motility. Prey are then invaded to form a "bdelloplast" in a type IV pilus-dependent process, and prey contents are digested, allowing Bdellovibrio growth and septation. After septation, Bdellovibrio flagellar motility resumes inside the prey bdelloplast prior to its lysis and escape of Bdellovibrio progeny. Bdellovibrio can also grow slowly outside prey as long flagellate host-independent (HI) cells, cultured on peptone-rich media. The B. bacteriovorus HD100 genome encodes three pairs of MotAB flagellar motor proteins, each of which could potentially form an inner membrane ion channel, interact with the FliG flagellar rotor ring, and produce flagellar rotation. In 2004, Flannagan and coworkers (R. S. Flannagan, M. A. Valvano, and S. F. Koval, Microbiology 150:649-656, 2004) used antisense RNA and green fluorescent protein (GFP) expression to downregulate a single Bdellovibrio motA gene and reported slowed release from the bdelloplast and altered motility of the progeny. Here we inactivated each pair of motAB genes and found that each pair contributes to motility, both predatorily, inside the bdelloplast and during HI growth; however, each pair was dispensable, and deletion of no pair abolished motility totally. Driving-ion studies with phenamil, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and different pH and sodium conditions indicated that all Mot pairs are proton driven, although the sequence similarities of each Mot pair suggests that some may originate from halophilic species. Thus, Bdellovibrio is a "dedicated motorist," retaining and expressing three pairs of mot genes.
Ann file
T1 Species 36 62 Bdellovibrio bacteriovorus
N1 Reference T1 Taxonomy:959
T2 Species 180 206 Bdellovibrio bacteriovorus
N2 Reference T2 Taxonomy:959
T3 Out-of-scope 490 502 Bdellovibrio
T4 Out-of-scope 542 554 Bdellovibrio
T5 Out-of-scope 643 655 Bdellovibrio
T6 Out-of-scope 665 677 Bdellovibrio
T7 Species 796 818 B. bacteriovorus HD100
T8 Out-of-scope 1232 1244 Bdellovibrio
T9 Out-of-scope 1874 1886 Bdellovibrio
N7 Reference T7 Taxonomy:264462 Bdellovibrio bacteriovorus HD100
N3 Reference T3 Taxonomy:1825604 Cotterillia
N10 Reference T3 Taxonomy:958 Bdellovibrio
N4 Reference T4 Taxonomy:1825604 Cotterillia
N11 Reference T4 Taxonomy:958 Bdellovibrio
N5 Reference T5 Taxonomy:1825604 Cotterillia
N12 Reference T5 Taxonomy:958 Bdellovibrio
N6 Reference T6 Taxonomy:1825604 Cotterillia
N13 Reference T6 Taxonomy:958 Bdellovibrio
N8 Reference T8 Taxonomy:1825604 Cotterillia
N14 Reference T8 Taxonomy:958 Bdellovibrio
N9 Reference T9 Taxonomy:1825604 Cotterillia
N15 Reference T9 Taxonomy:958 Bdellovibrio