21199018
not annotated - annotated - LINNAEUS only
Analysis of expressed sequence tags from Maize mosaic rhabdovirus-infected gut tissues of Peregrinus maidis reveals the presence of key components of insect innate immunity.
The corn planthopper, Peregrinus maidis, causes direct feeding damage to plants and transmits Maize mosaic rhabdovirus (MMV) in a persistent-propagative manner. MMV must cross several insect tissue layers for successful transmission to occur, and the gut serves as an important barrier for rhabdovirus transmission. In order to facilitate the identification of proteins that may interact with MMV either by facilitating acquisition or responding to virus infection, we generated and analysed the gut transcriptome of P. maidis. From two normalized cDNA libraries, we generated a P. maidis gut transcriptome composed of 20,771 expressed sequence tags (ESTs). Assembly of the sequences yielded 1860 contigs and 14,032 singletons, and biological roles were assigned to 5793 (36%). Comparison of P. maidis ESTs with other insect amino acid sequences revealed that P. maidis shares greatest sequence similarity with another hemipteran, the brown planthopper Nilaparvata lugens. We identified 202 P. maidis transcripts with putative homology to proteins associated with insect innate immunity, including those implicated in the Toll, Imd, JAK/STAT, Jnk and the small-interfering RNA-mediated pathways. Sequence comparisons between our P. maidis gut EST collection and the currently available National Center for Biotechnology Information EST database collection for Ni. lugens revealed that a pathogen recognition receptor in the Imd pathway, peptidoglycan recognition protein-long class (PGRP-LC), is present in these two members of the family Delphacidae; however, these recognition receptors are lacking in the model hemipteran Acyrthosiphon pisum. In addition, we identified sequences in the P. maidis gut transcriptome that share significant amino acid sequence similarities with the rhabdovirus receptor molecule, acetylcholine receptor (AChR), found in other hosts. This EST analysis sheds new light on immune response pathways in hemipteran guts that will be useful for further dissecting innate defence response pathways to rhabdovirus infection.
Ann file
T1 Species 41 65 Maize mosaic rhabdovirus
N1 Reference T1 Taxonomy:279896
T2 Species 90 107 Peregrinus maidis
N2 Reference T2 Taxonomy:222432
T3 Species 180 196 corn planthopper
N3 Reference T3 Taxonomy:222432
T4 Species 198 215 Peregrinus maidis
N4 Reference T4 Taxonomy:222432
T5 Species 270 294 Maize mosaic rhabdovirus
N5 Reference T5 Taxonomy:279896
T6 Species 337 340 MMV
N6 Reference T6 Taxonomy:279896
T7 Species 569 572 MMV
N7 Reference T7 Taxonomy:279896
T8 Species 693 702 P. maidis
N8 Reference T8 Taxonomy:222432
T9 Species 755 764 P. maidis
N9 Reference T9 Taxonomy:222432
T10 Species 968 977 P. maidis
N10 Reference T10 Taxonomy:222432
T11 Species 1036 1045 P. maidis
N11 Reference T11 Taxonomy:222432
T12 Species 1111 1128 brown planthopper
N12 Reference T12 Taxonomy:108931
T13 Species 1129 1147 Nilaparvata lugens
N13 Reference T13 Taxonomy:108931
T14 Species 1167 1176 P. maidis
N14 Reference T14 Taxonomy:222432
T15 Species 1405 1414 P. maidis
N15 Reference T15 Taxonomy:222432
T16 Species 1536 1546 Ni. lugens
N16 Reference T16 Taxonomy:108931
T17 Species 1801 1820 Acyrthosiphon pisum
N17 Reference T17 Taxonomy:7029
T18 Species 1866 1875 P. maidis
N18 Reference T18 Taxonomy:222432
T19 Species 296 299 MMV