21205278
not annotated - annotated - LINNAEUS only
Molecular characterization of three genes encoding aminopeptidases N in the poplar leaf beetle Chrysomela tremulae.
Three genes encoding proteins showing sequence similarity and features typical of insect APNs were characterized in C. tremulae and designed as CtAPN1, CtAPN2 and CtAPN3. Expression analysis of the three C. tremulae APN genes showed that CtAPN2 transcript is more abundant in the fat body, whereas both CtAPN1 and CtAPN3 are specifically expressed in the midgut. Despite a similar genomic organization, lepidopteran and coleopteran APNs are phylogenetically distant, suggesting that APN gene duplication events occurred after these two insect orders split. Sequence and expression comparisons of CtAPN1, CtAPN2 and CtAPN3 cDNAs in a C. tremulae Bacillus thuringiensis (Bt)-susceptible and in a Bt-resistant strain did not show any polymorphism at the amino acid level or difference at the transcription level.
Ann file
T1 Species 76 94 poplar leaf beetle
N1 Reference T1 Taxonomy:63687
T2 Species 95 114 Chrysomela tremulae
N2 Reference T2 Taxonomy:63687
T3 Species 234 245 C. tremulae
N3 Reference T3 Taxonomy:63687
T4 Species 322 333 C. tremulae
N4 Reference T4 Taxonomy:63687
T5 Species 751 762 C. tremulae
N5 Reference T5 Taxonomy:63687
T6 Species 763 785 Bacillus thuringiensis
N6 Reference T6 Taxonomy:1428
T7 Species 787 789 Bt
N7 Reference T7 Taxonomy:1428
T8 Species 812 814 Bt
N8 Reference T8 Taxonomy:1428