21281727
not annotated - annotated - LINNAEUS only
Identification and characterization of putative osmosensors, HwSho1A and HwSho1B, from the extremely halotolerant black yeast Hortaea werneckii.
In Saccharomyces cerevisiae, the Sho1 protein is one of two potential osmosensors that can activate the kinase cascade of the HOG pathway in response to increased extracellular osmolarity. Two novel SHO1-like genes, HwSHO1A and HwSHO1B, have been cloned from the saltern-inhabiting, extremely halotolerant black yeast Hortaea werneckii. The HwSho1 protein isoforms are 93.8% identical in their amino-acid sequences, and have a conserved SH3 domain. When the HwSHO1 genes were transferred into S. cerevisae cells lacking the SHO1 gene, both of the HwSho1 isoforms fully complemented the function of the native S. cerevisiae Sho1 protein. Through microscopic and biochemical validation, we demonstrate that in S. cerevisiae, both of the HwSho1 proteins have characteristic subcellular localizations similar to the S. cerevisiae Sho1 protein, and they can both activate the HOG pathway under conditions of osmotic stress. To a lower extent, crosstalk to the mating pathway expressing HwSho1 proteins is conserved in the PBS2 deleted S. cerevisiae strain. These data show that the HwSho1 proteins from H. werneckii are true functional homologs of the Sho1 protein of S. cerevisiae.
Ann file
T1 Species 126 143 Hortaea werneckii
N1 Reference T1 Taxonomy:91943
T2 Species 150 174 Saccharomyces cerevisiae
N2 Reference T2 Taxonomy:4932
T3 Species 465 482 Hortaea werneckii
N3 Reference T3 Taxonomy:91943
T4 Species 640 652 S. cerevisae
N4 Reference T4 Taxonomy:4932
T5 Species 756 769 S. cerevisiae
N5 Reference T5 Taxonomy:4932
T6 Species 855 868 S. cerevisiae
N6 Reference T6 Taxonomy:4932
T7 Species 959 972 S. cerevisiae
N7 Reference T7 Taxonomy:4932
T8 Species 1177 1190 S. cerevisiae
N8 Reference T8 Taxonomy:4932
T9 Species 1245 1257 H. werneckii
N9 Reference T9 Taxonomy:91943
T10 Species 1310 1323 S. cerevisiae
N10 Reference T10 Taxonomy:4932
T11 Out-of-scope 114 125 black yeast
T12 Out-of-scope 453 464 black yeast
N11 Reference T11 Taxonomy:34395 Chaetothyriales
N12 Reference T12 Taxonomy:34395 Chaetothyriales