21282046
not annotated - annotated - LINNAEUS only
Polymorphism of Paramecium pentaurelia (Ciliophora, Oligohymenophorea) strains revealed by rDNA and mtDNA sequences.
Paramecium pentaurelia is one of 15 known sibling species of the Paramecium aurelia complex. It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing genetic polymorphism within P. pentaurelia including new strains recently found in Poland (originating from two water bodies, different years, seasons, and clones of one strain) as well as strains collected from distant habitats (USA, Europe, Asia), and strains representing other species of the complex. We compared two DNA fragments: partial sequences (349 bp) of the LSU rDNA and partial sequences (618 bp) of cytochrome B gene. A correlation between the geographical origin of the strains and the genetic characteristics of their genotypes was not observed. Different genotypes were found in Kraksigmaw in two types of water bodies (Opatkowice-natural pond; Jordan's Park-artificial pond). Haplotype diversity within a single water body was not recorded. Likewise, seasonal haplotype differences between the strains within the artificial water body, as well as differences between clones originating from one strain, were not detected. The clustering of some strains belonging to different species was observed in the phylogenies.
Ann file
T1 Species 16 38 Paramecium pentaurelia
N1 Reference T1 Taxonomy:43138
T2 Species 119 141 Paramecium pentaurelia
N2 Reference T2 Taxonomy:43138
T3 Species 445 459 P. pentaurelia
N3 Reference T3 Taxonomy:43138
T4 Species 184 202 Paramecium aurelia
N4 Reference T4 Taxonomy:5889 Paramecium aurelia