21295520
not annotated - annotated - LINNAEUS only
Increased sampling reveals novel lineages of Entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection.
To expand the representation for phylogenetic analysis, ten additional complete Entamoeba small-subunit rRNA gene sequences were obtained from humans, non-human primates, cattle and a tortoise. For some novel sequences no corresponding morphological data were available, and we suggest that these organisms should be referred to as ribosomal lineages (RL) rather than being assigned species names at present. To investigate genetic diversity and host specificity of selected Entamoeba species, a total of 91 new partial small subunit rRNA gene sequences were obtained, including 49 from Entamoeba coli, 18 from Entamoeba polecki, and 17 from Entamoeba hartmanni. We propose a new nomenclature for significant variants within established Entamoeba species. Based on current data we propose that the uninucleated-cyst-producing Entamoeba infecting humans is called Entamoeba polecki and divided into four subtypes (ST1-ST4) and that Entamoeba coli is divided into two subtypes (ST1-ST2). New hosts for several species were detected and, while host specificity and genetic diversity of several species remain to be clarified, it is clear that previous reliance on cultivated material has given us a misleading and incomplete picture of variation within the genus Entamoeba.
Ann file
T1 Species 294 300 humans
N1 Reference T1 Taxonomy:9606
T2 Species 322 328 cattle
N2 Reference T2 Taxonomy:9913
T3 Species 738 752 Entamoeba coli
N3 Reference T3 Taxonomy:110766
T4 Species 762 779 Entamoeba polecki
N4 Reference T4 Taxonomy:110769
T5 Species 793 812 Entamoeba hartmanni
N5 Reference T5 Taxonomy:110767
T6 Species 997 1003 humans
N6 Reference T6 Taxonomy:9606
T7 Species 1014 1031 Entamoeba polecki
N7 Reference T7 Taxonomy:110769
T8 Species 1082 1096 Entamoeba coli
N8 Reference T8 Taxonomy:110766
T9 Out-of-scope 302 320 non-human primates