21333593
not annotated - annotated - LINNAEUS only
Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus (Chlorophyta).
A detailed comparative examination of microtubule (MT) organization in interphase and dividing cells of Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus was made using tubulin immunofluorescence and transmission electron microscopy (TEM). During interphase all the species bear a well-organized cortical MT system, consisting of parallel bundles with different orientations. In Uronema sp. the cortical MT bundles are longitudinally oriented, whereas in the other species they are in transverse orientation to the axis of the cells. Considerable differences in MT organization were also observed during stages of mitosis, mainly preprophase, as well as cytokinesis. In Uronema sp., a particular radial MT assembly is organized during preprophase-early prophase, which was not observed in the other species. In Stichococcus a fine MT ring surrounded the nucleus during preprophase and prophase. An MT ring, together with single cytoplasmic MTs, was also found associated with the developing diaphragm during cytokinesis in Stichococcus. A phycoplast participates in cytokinesis in Uronema sp., but not in the other species. In Uronema sp. the centrosome functions as a microtubule organizing center (MTOC) during mitosis, but not during interphase and cytokinesis. The phylogenetic significance of these differences is discussed in combination with SSU/ITS sequencing and other, existing molecular data.
Ann file
T1 Species 104 127 Klebsormidium flaccidum
N1 Reference T1 Taxonomy:3175
T2 Species 129 145 K. subtilissimum
N2 Reference T2 Taxonomy:184584
T3 Species 147 170 Stichococcus bacillaris
N3 Reference T3 Taxonomy:37433
T4 Species 175 189 S. chloranthus
N4 Reference T4 Taxonomy:413990
T5 Species 324 347 Klebsormidium flaccidum
N5 Reference T5 Taxonomy:3175
T6 Species 349 365 K. subtilissimum
N6 Reference T6 Taxonomy:184584
T7 Species 367 390 Stichococcus bacillaris
N7 Reference T7 Taxonomy:37433
T8 Species 395 409 S. chloranthus
N8 Reference T8 Taxonomy:413990