21382108

not annotated - annotated - LINNAEUS only

Responses of the pea aphid transcriptome to infection by facultative symbionts.

Serratia symbiotica is a facultative symbiont of pea aphids (Acyrthosiphon pisum) that provides tolerance to heat stress. Although the phenotypic effects of facultative symbionts upon hosts have been studied in some detail, little is known about the molecular and genomic basis of these interactions. Previous studies show a large impact of S. symbiotica upon the aphid metabolome. Whole-genome transcriptional profiling and next-generation sequencing demonstrated expression of 94% of RefSeq genes from the pea aphid genome, providing the largest dataset to date on aphid gene expression. However, only 28 genes showed changes in expression with S. symbiotica infection, and these changes were of small magnitude. No expression differences in genes involved in innate immunity in other insects were observed. Therefore, the large metabolic impact of S. symbiotica is most likely a result of metabolism of the symbiont itself, or of post-transcriptional modification of host gene expression. Although S. symbiotica has a major influence on its host's metabolome and resistance to heat, it induces little change in gene expression in its host.



Ann file

T1	Species 17 26	pea aphid

N1 Reference T1 Taxonomy:7029

T2 Species 81 100 Serratia symbiotica

N2 Reference T2 Taxonomy:138074

T3 Species 130 140 pea aphids

N3 Reference T3 Taxonomy:7029

T4 Species 142 161 Acyrthosiphon pisum

N4 Reference T4 Taxonomy:7029

T5 Species 422 435 S. symbiotica

N5 Reference T5 Taxonomy:138074

T6 Species 589 598 pea aphid

N6 Reference T6 Taxonomy:7029

T7 Species 728 741 S. symbiotica

N7 Reference T7 Taxonomy:138074

T8 Species 932 945 S. symbiotica

N8 Reference T8 Taxonomy:138074

T9 Species 1082 1095 S. symbiotica

N9 Reference T9 Taxonomy:138074