21600998
not annotated - annotated - LINNAEUS only
The cell cycle gene MoCDC15 regulates hyphal growth, asexual development and plant infection in the rice blast pathogen Magnaporthe oryzae.
Rice blast, caused by the pathogen Magnaporthe oryzae, is a serious hindrance to rice production and has emerged as an important model for the characterization of molecular mechanisms relevant to pathogenic development in plants. Similar to other pathogenic fungi, conidiation plays a central role in initiation of M.oryzae infection and spread over a large area. However, relatively little is known regarding the molecular mechanisms that underlie conidiation in M. oryzae. To better characterize these mechanisms, we identified a conidiation-defective mutant, ATMT0225B6 (MoCDC15(T-DNA)), in which a T-DNA insertion disrupted a gene that encodes a homolog of fission yeast cdc15, and generated a second strain containing a disruption in the same allele (DeltaMoCDC15(T-DNA)). The cdc15 gene has been shown to act as a coordinator of the cell cycle in yeast. Functional analysis of the MoCDC15(T-DNA) and DeltaMoCDC15(T-DNA) mutants revealed that MoCDC15 is required for conidiation, preinfection development and pathogenicity in M. oryzae. Conidia from these mutants were viable, but failed to adhere to hydrophobic surface, a crucial step required for subsequent pathogenic development. All phenotypic defects observed in mutants were rescued in a strain complemented with wild type MoCDC15. Together, these data indicate that MoCDC15 functions as a coordinator of several biological processes important for pathogenic development in M. oryzae.
Ann file
T1 Species 100 104 rice
T2 Species 120 138 Magnaporthe oryzae
N2 Reference T2 Taxonomy:318829
T3 Species 177 195 Magnaporthe oryzae
N3 Reference T3 Taxonomy:318829
T4 Species 223 227 rice
N4 Reference T4 Taxonomy:4530
T5 Species 457 465 M.oryzae
N5 Reference T5 Taxonomy:318829
T6 Species 606 615 M. oryzae
N6 Reference T6 Taxonomy:318829
T7 Species 704 714 ATMT0225B6
N7 Reference T7 Taxonomy:318829
T8 Species 716 730 MoCDC15(T-DNA)
N8 Reference T8 Taxonomy:318829
T10 Out-of-scope 995 1000 yeast
T11 Species 1173 1182 M. oryzae
N11 Reference T11 Taxonomy:318829
T12 Species 1418 1435 wild type MoCDC15
N12 Reference T12 Taxonomy:318829
T13 Species 1579 1588 M. oryzae
N13 Reference T13 Taxonomy:318829
T14 Species 142 146 Rice
N14 Reference T14 Taxonomy:4530 Oryza sativa
#1 AnnotatorNotes T12 TODO check span
N10 Reference T10 Taxonomy:147537 Saccharomycotina
T9 Out-of-scope 803 816 fission yeast
N9 Reference T9 Taxonomy:4894 Schizosaccharomycetaceae
N1 Reference T1 Taxonomy:4530 Oryza sativa