21641778
not annotated - annotated - LINNAEUS only
Suitability of different media for in vitro cultivation of the ruminal protozoa species Entodinium caudatum, Eudiplodinium maggii, and Epidinium ecaudatum.
Three protozoal cultivation media were tested to determine the medium which best facilitated growth and viability of key B-type ciliates isolated from the sheep rumen. Entodinium caudatum and Eudiplodinium maggii were grown anaerobically in 50-ml flasks for 32 days in Caudatum-type (C), Kisidayova (K) or Dehority (M) medium. On day 32, in media K and M, E. caudatum cell counts were high with 5.6x10(3) and 7.8x10(3) mL(-1), respectively, and the proportion of dead cells was low with 0.6 and 1.4%, respectively. E. maggii concentrations when grown in medium M and C were 2.7x10(3) and 2.4x10(3) mL(-1), respectively, with 3.9 and 14.1% dead cells. Medium M, which favoured growth of both protozoa species, was tested again and Epidinium ecaudatum was included. Protozoa were grown for a 4-month period and samples were taken in the last two months on days 1, 7, 35 and 57. Average cell concentrations were 10.0, 0.8 and 0.5x10(3) mL(-1) for E. caudatum, E. maggii, and E. ecaudatum, respectively. In conclusion, medium M would appear to be the best choice for cultivating these three species in one medium.
Ann file
T1 Species 88 107 Entodinium caudatum
N1 Reference T1 Taxonomy:47911
T2 Species 109 129 Eudiplodinium maggii
N2 Reference T2 Taxonomy:47892
T3 Species 135 154 Epidinium ecaudatum
N3 Reference T3 Taxonomy:300017
T4 Species 326 345 Entodinium caudatum
N4 Reference T4 Taxonomy:47911
T5 Species 350 370 Eudiplodinium maggii
N5 Reference T5 Taxonomy:47892
T6 Species 514 525 E. caudatum
N6 Reference T6 Taxonomy:47911
T7 Species 673 682 E. maggii
N7 Reference T7 Taxonomy:47892
T8 Species 888 907 Epidinium ecaudatum
N8 Reference T8 Taxonomy:300017
T9 Species 1102 1113 E. caudatum
N9 Reference T9 Taxonomy:47911
T10 Species 1115 1124 E. maggii
N10 Reference T10 Taxonomy:47892
T11 Species 1130 1142 E. ecaudatum
N11 Reference T11 Taxonomy:300017
T12 Species 313 318 sheep
N12 Reference T12 Taxonomy:9940 Ovis aries