21668550
not annotated - annotated - LINNAEUS only
Compatible interaction with its rice host leads to enhanced expression of the gamma subunit of oligosaccharyl transferase in the Asian rice gall midge, Orseolia oryzae.
The Asian rice gall midge, Orseolia oryzae, is a fast evolving, damaging pest of rice. Understanding the underlying molecular mechanism of interaction between the gall midge and rice will help in devising strategies to control and manage the pest. The present study aims to identify rice-responsive genes in the gall midge that aid pest survival. The abundance of transcripts coding for enzymes related to glycosylation, in a cDNA library prepared from maggots of the rice gall midge feeding on susceptible hosts, indicated their probable involvement in the gall midge-rice interaction. Hence, a full-length transcript for a gamma subunit of the oligosaccharyl transferase gene (OoOST) from the gall midge was cloned and characterized. It has 72% similarity to its orthologue cloned from Aedes aegypti. Tissue-specific analysis of the expression of OoOST revealed an increase (> sevenfold) in the transcripts of the gene in the salivary glands of maggots in susceptible plants when compared with the transcript level in the salivary glands of maggots feeding on resistant hosts. Using quantitative PCR, performed on different developmental stages of the maggots in two susceptible and two resistant hosts, we observed similar expression patterns (i.e. overexpression in the compatible interaction). These results indicate the involvement of OoOST in maggot survival and establishment in the susceptible host. In order to identify polymorphism in the gene, OoOST was cloned from three gall midge biotypes GMB1, GMB4 and GMB4M.
Ann file
T1 Species 129 150 Asian rice gall midge
N1 Reference T1 Taxonomy:33408
T2 Species 152 167 Orseolia oryzae
N2 Reference T2 Taxonomy:33408
T3 Species 175 196 Asian rice gall midge
N3 Reference T3 Taxonomy:33408
T4 Species 198 213 Orseolia oryzae
N4 Reference T4 Taxonomy:33408
T5 Species 959 972 Aedes aegypti
N5 Reference T5 Taxonomy:7159
T6 Species 32 36 rice
T7 Species 252 256 rice
T8 Species 349 353 rice
T9 Species 334 344 gall midge
N6 Reference T9 Taxonomy:33408 Orseolia oryzae
T10 Species 454 458 rice
T11 Species 639 654 rice gall midge
N7 Reference T11 Taxonomy:33408 Orseolia oryzae
T12 Species 729 739 gall midge
N8 Reference T12 Taxonomy:33408 Orseolia oryzae
T13 Species 740 744 rice
N9 Reference T6 Taxonomy:4530 Oryza sativa
N10 Reference T7 Taxonomy:4530 Oryza sativa
N11 Reference T8 Taxonomy:4530 Oryza sativa
N12 Reference T10 Taxonomy:4530 Oryza sativa
N13 Reference T13 Taxonomy:4530 Oryza sativa