21711401
not annotated - annotated - LINNAEUS only
Identification and characterization of the cysteine protease inhibitor gene MdCPI from Musca domestica.
Cysteine proteinase inhibitors (CPIs) are involved in many vital cellular processes such as signalling pathways, apoptosis, immune response and development; however, no CPIs have yet been reported from the housefly Musca domestica. Here we report the isolation and characterization of a housefly CPI gene designated MdCPI. The gene contains an open reading frame of 357 bp encoding a protein of 118 amino acid residues with a putative signal peptide of 17 amino acid residues. Protein alignment demonstrated a high homology to that of Sarcophaga crassipalpis (identity = 51%). Phylogenetic analysis suggested that all CPIs from dipterans, including the housefly, belong to the I25A family and may be descended from a single common ancestor. The gene was expressed in and purified from Escherichia coli. Biochemical studies showed that MdCPI exerts an inhibiting function on papain, which is a classical assay to confirm CPIs. Real-time quantitative PCR and immunolocalization analysis revealed that MdCPI is specifically expressed in haemocytes and fat bodies. It is highly down-regulated in larvae and markedly up-regulated in the pupal stage, suggesting that it may be related to development.
Ann file
T1 Species 87 102 Musca domestica
N1 Reference T1 Taxonomy:7370
T2 Species 312 320 housefly
N2 Reference T2 Taxonomy:7370
T3 Species 321 336 Musca domestica
N3 Reference T3 Taxonomy:7370
T4 Species 393 401 housefly
N4 Reference T4 Taxonomy:7370
T5 Species 641 664 Sarcophaga crassipalpis
N5 Reference T5 Taxonomy:59312
T6 Species 759 767 housefly
N6 Reference T6 Taxonomy:7370
T7 Species 891 907 Escherichia coli
N7 Reference T7 Taxonomy:562