21807108
not annotated - annotated - LINNAEUS only
Fluorescent protein fusions in Candida guilliermondii.
Candida guilliermondii is an emerging fungal agent of candidiasis often associated with oncology patients. This yeast also remains a promising biotechnological model for the industrial production of value-added metabolites. In the present study, we developed a recipient strain as well as a set of plasmids for construction of fluorescent protein (FP) fusions in this species. We demonstrated that C. guilliermondii phosphoglycerate kinase transcription-regulating sequences allow a constitutive expression of codon-optimized green, cyan, yellow and mCherry FP genes in C. guilliermondii cells and the fluorescence signal could be directly observed at the colony and blastospore level by epifluorescence microcopy. To illustrate differential targeting of the FPs into specified cellular compartments, we studied and validated the expected subcellular localization of various C. guilliermondii predicted proteins fused to FPs. Furthermore, co-expression experiments of various couples of FP-tagged C. guilliermondii predicted proteins in the same cell showed that the fluorescence of each FP could be detected independently, providing firm evidences that YFP/CFP and GFP/mCherry pairs can be used for dual labeling in C. guilliermondii cells. This technical advance will facilitate future studies of protein co-expression and co-localization in C. guilliermondii and will give precious help for elucidating new molecular events supporting pathogenicity, antifungal resistance and for exploring the potential of yeast metabolic engineering.
Ann file
T1 Species 31 53 Candida guilliermondii
N1 Reference T1 Taxonomy:4929
T2 Species 57 79 Candida guilliermondii
N2 Reference T2 Taxonomy:4929
T3 Out-of-scope 169 174 yeast
T4 Species 455 472 C. guilliermondii
N4 Reference T4 Taxonomy:4929
T5 Species 627 644 C. guilliermondii
N5 Reference T5 Taxonomy:4929
T6 Species 932 949 C. guilliermondii
N6 Reference T6 Taxonomy:4929
T7 Species 1054 1071 C. guilliermondii
N7 Reference T7 Taxonomy:4929
T8 Species 1274 1291 C. guilliermondii
N8 Reference T8 Taxonomy:4929
T9 Species 1401 1418 C. guilliermondii
N9 Reference T9 Taxonomy:4929
T10 Out-of-scope 1567 1572 yeast
N3 Reference T3 Taxonomy:147537 Saccharomycotina
N10 Reference T10 Taxonomy:147537 Saccharomycotina