21815956

not annotated - annotated - LINNAEUS only

Insertion of an intact CR1 retrotransposon in a cadherin gene linked with Bt resistance in the pink bollworm, Pectinophora gossypiella.

Three mutations in the Pectinophora gossypiella cadherin gene PgCad1 are linked with resistance to Bacillus thuringiensis (Bt) toxin Cry1Ac. Here we show that the r3 mutation entails recent insertion into PgCad1 of an active chicken repeat (CR1) retrotransposon, designated CR1-1_Pg. Unlike most other CR1 elements, CR1-1_Pg is intact, transcribed by a flanking promoter, contains target site duplications and has a relatively low number of copies. Examination of transcripts from the PgCad1 locus revealed that CR1-1_Pg disrupts both the cadherin protein and a long noncoding RNA of unknown function. Together with previously reported data, these findings show that transposable elements disrupt eight of 12 cadherin alleles linked with resistance to Cry1Ac in three lepidopteran species, indicating that the cadherin locus is a common target for disruption by transposable elements.



Ann file

T1	Species 95 108	pink bollworm

N1 Reference T1 Taxonomy:13191

T2 Species 110 134 Pectinophora gossypiella

N2 Reference T2 Taxonomy:13191

T3 Species 161 185 Pectinophora gossypiella

N3 Reference T3 Taxonomy:13191

T4 Species 237 259 Bacillus thuringiensis

N4 Reference T4 Taxonomy:1428

T5 Species 261 263 Bt

N5 Reference T5 Taxonomy:1428

T6 Species 363 370 chicken

N6 Reference T6 Taxonomy:9031

#1 AnnotatorNotes T6 TODO check

T7 Species 74 76 Bt

N7 Reference T7 Taxonomy:1428