21840412
not annotated - annotated - LINNAEUS only
Expression and functional characterisation of TNC, a high-affinity nickel transporter from Neurospora crassa.
Our previous in silico studies identified a high-affinity nickel transporter,TNC, from the metal transportome of Neurospora crassa. A knockout mutant of the tnc gene in N. crassa failed to transport nickel, showed phenotypic growth defects and diminished urease activity under physiological levels of nickel. Transport assays conducted in wild type and knockout mutant strains showed that TNC transports nickel withhigh affinity but exhibits selectivity for other transition metal ions like cobalt. Heterologous complementation of Schizosaccharomyces pombe nickel uptake mutant by TNC further substantiates its nickel transport function. Transcriptional analysis of the nickel transporter encoding gene, tnc in N. crassa by qRT-PCR showed its constitutive expression in various phases of its life cycle. However, levels of the corresponding protein TNC were down-regulated only by increasing the nickel, but not cobalt concentration in the media. Immunolocalisation data suggested that TNC is distributed in the plasma membrane of N. crassa. Thus, the present study establishes TNC as a functional plasma membrane nickel transporter necessary for physiological acquisition of nickel in the multicellular fungi N. crassa.
Ann file
T1 Species 91 108 Neurospora crassa
N1 Reference T1 Taxonomy:5141
T2 Species 225 242 Neurospora crassa
N2 Reference T2 Taxonomy:5141
T3 Species 281 290 N. crassa
N3 Reference T3 Taxonomy:5141
T4 Species 643 668 Schizosaccharomyces pombe
N4 Reference T4 Taxonomy:4896
T5 Species 823 832 N. crassa
N5 Reference T5 Taxonomy:5141
T6 Species 1143 1152 N. crassa
N6 Reference T6 Taxonomy:5141
T7 Species 1322 1331 N. crassa
N7 Reference T7 Taxonomy:5141