21840413
not annotated - annotated - LINNAEUS only
The effects of dsRNA mycoviruses on growth and murine virulence of Aspergillus fumigatus.
Some isolates of the opportunistic human pathogenic fungus Aspergillus fumigatus are known to be infected with mycoviruses. The dsRNA genomes of two of these mycoviruses, which include a chrysovirus and a partitivirus, have been completely sequenced and an RT-PCR assay for the viruses has been developed. Through curing virus-infected A. fumigatus isolates by cycloheximide treatment and transfecting virus-free isolates with purified virus, as checked by RT-PCR, isogenic virus-free and virus-infected lines of the fungus were generated whose phenotypes and growth have been directly compared. Mycovirus infection of A. fumigatus with either the chrysovirus or the partitivirus resulted in significant aberrant phenotypic alterations and attenuation of growth of the fungus but had no effect on susceptibility to common antifungals. Chrysovirus infection of A. fumigatus caused no significant alterations to murine pathogenicity.
Ann file
T1 Species 67 88 Aspergillus fumigatus
N1 Reference T1 Taxonomy:746128
T2 Species 127 132 human
N2 Reference T2 Taxonomy:9606
T3 Species 151 172 Aspergillus fumigatus
N3 Reference T3 Taxonomy:746128
T4 Species 428 440 A. fumigatus
N4 Reference T4 Taxonomy:746128
T5 Species 711 723 A. fumigatus
N5 Reference T5 Taxonomy:746128
T6 Species 952 964 A. fumigatus
N6 Reference T6 Taxonomy:746128