21906194
not annotated - annotated - LINNAEUS only
Prolixicin: a novel antimicrobial peptide isolated from Rhodnius prolixus with differential activity against bacteria and Trypanosoma cruzi.
We identified and characterized the activity of prolixicin, a novel antimicrobial peptide (AMP) isolated from the hemipteran insect, Rhodnius prolixus. Sequence analysis reveals one region of prolixicin that may be related to the diptericin/attacin family of AMPs. Prolixicin is an 11-kDa peptide containing a putative 21 amino acid signal peptide, two putative phosphorylation sites and no glycosylation sites. It is produced by both adult fat body and midgut tissues in response to bacterial infection of the haemolymph or the midgut. Unlike most insect antibacterial peptides, the prolixicin gene does not seem to be regulated by NF-kappaB binding sites, but its promoter region contains several GATA sites. Recombinant prolixicin has strong activity against the Gram-negative bacterium Escherichia coli and differential activity against several Gram-negative and Gram-positive bacteria. No significant toxicity was demonstrated against Trypanosoma cruzi, the human parasite transmitted by R. prolixus.
Ann file
T1 Species 56 73 Rhodnius prolixus
N1 Reference T1 Taxonomy:13249
T2 Species 122 139 Trypanosoma cruzi
N2 Reference T2 Taxonomy:5693
T3 Species 276 293 Rhodnius prolixus
N3 Reference T3 Taxonomy:13249
T4 Species 933 949 Escherichia coli
N4 Reference T4 Taxonomy:562
T5 Species 1083 1100 Trypanosoma cruzi
N5 Reference T5 Taxonomy:5693
T6 Species 1106 1111 human
N6 Reference T6 Taxonomy:9606
T7 Species 1136 1147 R. prolixus
N7 Reference T7 Taxonomy:13249