21907298
not annotated - annotated - LINNAEUS only
Galleria mellonella as model host for the trans-kingdom pathogen Fusarium oxysporum.
Fusarium oxysporum, the causal agent of vascular wilt disease, affects a wide range of plant species and can produce disseminated infections in humans. F. oxysporum f. sp. lycopersici isolate FGSC 9935 causes disease both on tomato plants and immunodepressed mice, making it an ideal model for the comparative analysis of fungal virulence on plant and animal hosts. Here we tested the ability of FGSC 9935 to cause disease in the greater wax moth Galleria mellonella, an invertebrate model host that is widely used for the study of microbial human pathogens. Injection of living but not of heat-killed microconidia into the hemocoel of G. mellonella larvae resulted in dose-dependent killing both at 30^0C and at 37^0C. Fluorescence microscopy of larvae inoculated with a F. oxysporum transformant expressing GFP revealed hyphal proliferation within the hemocoel, interaction with G. mellonella hemocytes, and colonization of the killed insects by the fungus. Fungal gene knockout mutants previously tested in the tomato and immunodepressed mouse systems displayed a good correlation in virulence between the Galleria and the mouse model. Thus, Galleria represents a useful non-vertebrate infection model for studying virulence mechanisms of F. oxysporum on animal hosts.
Ann file
T1 Species 0 19 Galleria mellonella
N1 Reference T1 Taxonomy:7137
T2 Species 65 83 Fusarium oxysporum
N2 Reference T2 Taxonomy:5507
T3 Species 87 105 Fusarium oxysporum
N3 Reference T3 Taxonomy:5507
T4 Species 231 237 humans
N4 Reference T4 Taxonomy:9606
T5 Species 239 288 F. oxysporum f. sp. lycopersici isolate FGSC 9935
N5 Reference T5 Taxonomy:5507
T6 Species 312 318 tomato
N6 Reference T6 Taxonomy:4081
T7 Species 346 350 mice
N7 Reference T7 Taxonomy:10090
T8 Species 483 492 FGSC 9935
N8 Reference T8 Taxonomy:5507
T9 Species 517 533 greater wax moth
N9 Reference T9 Taxonomy:7137
T10 Species 534 553 Galleria mellonella
N10 Reference T10 Taxonomy:7137
T11 Species 629 634 human
N11 Reference T11 Taxonomy:9606
T12 Species 723 736 G. mellonella
N12 Reference T12 Taxonomy:7137
T13 Species 859 871 F. oxysporum
N13 Reference T13 Taxonomy:5507
T14 Species 968 981 G. mellonella
N14 Reference T14 Taxonomy:7137
T15 Species 1101 1107 tomato
N15 Reference T15 Taxonomy:4081
T16 Species 1128 1133 mouse
N16 Reference T16 Taxonomy:10090
T17 Species 1213 1218 mouse
N17 Reference T17 Taxonomy:10090
T18 Species 1329 1341 F. oxysporum
N18 Reference T18 Taxonomy:5507