22004876
not annotated - annotated - LINNAEUS only
Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance.
This study was conducted to assess evolutionary relationships, species diversity and trichothecene toxin potential of five Fusarium graminearum complex (FGSC) isolates identified as genetically novel during prior Fusarium head blight (FHB) surveys in Nepal and Louisiana. Results of a multilocus genotyping (MLGT) assay for B-trichothecene species determination indicated these isolates might represent novel species within the FGSC. GCPSR-based phylogenetic analyses of a 12-gene dataset, comprising portions of seven loci totaling 13.1kb of aligned DNA sequence data, provided strong support for the genealogical exclusivity of the Nepalese and Louisianan isolates. Accordingly, both species are formally recognized herein as novel FGSC species. Fusarium nepalense was resolved as the sister lineage of Fusarium ussurianum+Fusarium asiaticum within an Asian subclade of the FGSC. Fusarium louisianense was strongly supported as a reciprocally monophyletic sister of Fusarium gerlachii+F. graminearum, suggesting that this subclade might be endemic to North America. Multilocus Bayesian species tree analyses augment these results and provide evidence for a distinct lineage within F. graminearum predominately from the Gulf Coast of Louisiana. As predicted by the MLGT assay, mycotoxin analyses demonstrated that F. nepalense and F. louisianense could produce 15ADON and nivalenol, respectively, in planta. In addition, both species were only able to induce mild FHB symptoms on wheat in pathogenicity experiments.
Ann file
T1 Species 236 256 Fusarium graminearum
N1 Reference T1 Taxonomy:5518
T2 Species 266 270 FGSC
N2 Reference T2 Taxonomy:5518
T3 Species 541 545 FGSC
N3 Reference T3 Taxonomy:5518
T4 Species 847 851 FGSC
N4 Reference T4 Taxonomy:5518
T5 Species 861 879 Fusarium nepalense
T6 Species 918 937 Fusarium ussurianum
T7 Species 989 993 FGSC
N7 Reference T7 Taxonomy:5518
T8 Species 995 1016 Fusarium louisianense
N8 Reference T8 Taxonomy:5518
T10 Species 1296 1310 F. graminearum
N10 Reference T10 Taxonomy:5518
T11 Species 1428 1440 F. nepalense
N11 Reference T11 Taxonomy:5518
T12 Species 1445 1460 F. louisianense
N12 Reference T12 Taxonomy:5518
T13 Species 1594 1599 wheat
N13 Reference T13 Taxonomy:4565 Triticum aestivum
T14 Species 938 956 Fusarium asiaticum
T15 Species 1081 1099 Fusarium gerlachii
T16 Species 1100 1114 F. graminearum
N16 Reference T16 Taxonomy:5518
N6 Reference T6 Taxonomy:1284706 Fusarium ussurianum
N9 Reference T14 Taxonomy:282267 Fusarium asiaticum
N5 Reference T5 Taxonomy:1571107 Fusarium nepalense
N14 Reference T15 Taxonomy:569362 Fusarium gerlachii