22056521
not annotated - annotated - LINNAEUS only
Accumulation of P-bodies in Candida albicans under different stress and filamentous growth conditions.
Candida albicans is an opportunistic fungal pathogen that grows as budding yeast, pseudohyphal, and hyphal forms. In response to external signals, C. albicans switches rapidly among these forms. mRNA-containing cytoplasmic granules, termed processing bodies (P-bodies), have been reported to accumulate under various environmental stress conditions in diverse species from yeast to mammals. Here, we provide the first microscopic and genetic characterization of P-bodies in C. albicans. The core components of P-bodies, including the decapping machinery (Dcp2 and Dhh1), 5'-3' exoribonuclease (Kem1/Xrn1), and the P-body scaffolding protein (Edc3), were identified and their localizations with respect to P-bodies were demonstrated. Various growth conditions, including glucose deprivation, hyperosmotic stress, and heat stress, stimulated the accumulation of P-bodies. In addition, we observed P-body aggregation during hyphal development. The deletion mutant strain edc3/edc3 had a defect in filamentation and exhibited a dramatic reduction in the number of P-bodies. These results suggest that Edc3 plays an essential role in the assembly and maintenance of P-bodies in C. albicans, and that the switch to filamentous growth appears to accompany P-body accumulation.
Ann file
T1 Species 28 44 Candida albicans
N1 Reference T1 Taxonomy:5476
T2 Species 105 121 Candida albicans
N2 Reference T2 Taxonomy:5476
T3 Species 252 263 C. albicans
N3 Reference T3 Taxonomy:5476
T4 Out-of-scope 478 483 yeast
T5 Species 579 590 C. albicans
N5 Reference T5 Taxonomy:5476
T6 Species 1278 1289 C. albicans
N6 Reference T6 Taxonomy:5476
T7 Out-of-scope 172 185 budding yeast
N4 Reference T4 Taxonomy:147537 Saccharomycotina
N7 Reference T7 Taxonomy:4892 Saccharomycetales