22182607
not annotated - annotated - LINNAEUS only
Culture Independent Survey of the Microbiota of the Glassy-Winged Sharpshooter (Homalodisca vitripennis) Using 454 Pyrosequencing.
The glassy-winged sharpshooter, Homalodisca vitripennis (Germar), is an invasive pest that has spread across the southern and western United States. H. vitripennis is highly polyphagous and voracious, feeding on at least 100 plant species and consuming up to 100 times its weight in xylem fluid daily. The insect is a vector of the phytopathogen Xylella fastidiosa (Wells), which is the causative agent of Pierce's disease in grapevines. To evaluate the microbial flora associated with H. vitripennis, total DNA extracts from hemolymph, alimentary canal excretions, and whole insect bodies were subjected to 16S rDNA pyrosequencing using the bTEFAP methodology and the resulting sequences (370-520 bp in length) were compared with a curated high quality 16S database derived from GenBank http://www.ncbi.nlm.nih.gov. Species from the genera Wolbachia, Delftia (formerly Pseudomonas), Pectobacterium, Moraxella, Serratia, Bacillus, and many others were detected and a comprehensive picture of the microbiome associated with H. vitripennis was established. Some of the bacteria identified in this report are initial discoveries; providing a breadth of knowledge to the microbial flora of this insect pest can serve as a reservoir of information for developing biological control strategies.
Ann file
T1 Species 52 78 Glassy-Winged Sharpshooter
N1 Reference T1 Taxonomy:197043
T2 Species 80 103 Homalodisca vitripennis
N2 Reference T2 Taxonomy:197043
T3 Species 137 163 glassy-winged sharpshooter
N3 Reference T3 Taxonomy:197043
T4 Species 165 188 Homalodisca vitripennis
N4 Reference T4 Taxonomy:197043
T5 Species 282 296 H. vitripennis
N5 Reference T5 Taxonomy:197043
T6 Species 479 497 Xylella fastidiosa
N6 Reference T6 Taxonomy:2371
T7 Species 619 633 H. vitripennis
N7 Reference T7 Taxonomy:197043
T8 Species 1156 1170 H. vitripennis
N8 Reference T8 Taxonomy:197043